A shared-use facility that supports expression, purification, and analysis of research proteins by providing an array of instrumentation and consultation on strategies for protein production from small to large scale.
The Facility is managed by Dave Wood, Ph.D. (314-977-9220; firstname.lastname@example.org).
For a complete description of all available services, please see the Protein Core website.
A full-service facility that provides investigators with a variety of options for whole genome analysis via microarrays, high-throughput screening, immunoprecipitation, and sequencing strategies.
The Facility is managed by Dale Dorsett, Ph.D. (314-977-9218; email@example.com) and Audrey Watson (314-977-9252; firstname.lastname@example.org).
A state-of-the-art X-ray facility is available to researchers in the department for the collection of high-resolution data on macromolecular samples. The facility has a 0.8 kW MM007 Rigaku generator with VHF optics interfaced to an R-Axis IV++ imaging plate system and a 1.2 kW MM007HF Rigaku generator with VHF optics double ported with two R-Axis IV++ imaging plate systems. A Phoenix robotic instrumentation from Art Robbins Instruments is available for the preparation, screening and imaging of crystals.
The Facility is managed by Sergey Korolev, Ph.D. (314-977-9261; email@example.com).
The Department of Biochemistry and Molecular Biology maintains a wide variety of computing resources. These range from network and backup services to state-of-the-art high performance computing.
The most recent addition to the department is the Gemini Computing Cluster, a state-of-the-art, high performance computing cluster, leveraging modern technologies such as GPU computing capabilities. You can find out more information on the Gemini website.
Computational publications resulting from use of the Gemini Computing Cluster for some component of the work:
Funding resulting from use of the Gemini Computing Cluster for some component of the work:
The MicroTime 200 from Picoquant is a time-resolved confocal microscope with unique single molecule sensitivity. It includes an Olympus X71 inverted microscope-based system with 488, 532, and 640 nm picosecond pulsed lasers, four detection channels, and a piezo xyz scanner. Emission can be split according to color or polarization. Each collected photon is time-stamped both in nanoseconds timescale (from the laser pulse) and in seconds scale (from the beginning of the experiments), allowing flexible analyses of the collected data.
Typical uses of this instrument are for single molecule FRET, including Pulsed Interleaved Excitation (PIE) FRET. PIE interrogates each detected molecule with alternating donor and acceptor excitation with MHz speeds, allowing sorting of the molecules according the stoichiometry of donor and acceptor labels. FCS, FCCS, FLCS, TCSPC histogram, and photon-counting histogram (PCH) are examples of some additional analyses possible for freely diffusing molecules. Also, the following imaging measurement analyses are possible: Time-gated Fluorescence Intensity Imaging, Fluorescence Lifetime Imaging (FLIM), FLIM-FRET, Intensity FRET, and Anisotropy imaging.
The MicroTime 200 is managed by Nicola Pozzi, Ph.D. (314-977-9259; firstname.lastname@example.org).
The Olympus cellTIRF-4Line system allows ultra-sensitive, simultaneous multicolor Total Internal Reflection (TIRF) using four laser channels with independent beam paths. This inverted microscope-based system with cellTIRF laser illumination (491 nm, 561nm, and 640 nm) allows through-objective TIRF with software control of the depth of evanescent field, allowing the user to produce high-contrast images with minimal background noise for cell surface and single molecule studies.
An Andor IXON Ultra897 EMCCD camera and QV2 four-channel image splitter allow simultaneous recording of up to four color images of immobilized single molecules at up to 52 frames per second. Fast (1 ms) switching between lasers allows interrogating immobilized molecules with alternating two excitation wavelengths.
The Olympus cellTIRF4-Line system is managed by
Tomasz Heyduk, Ph.D. (314-977-9238; email@example.com).
The Bioinformatics Facility offers complete analysis of high-throughput expression data, including gene ontology enrichment analysis. We also offer next-generation genomic sequence assembly and variant analysis. We maintain an in-house MASCOT database for peptide mass searches, NCBI BLAST for custom database searching, and HMMer for protein domain searches. Custom script development is available for automation of protein and DNA sequence analysis. We have access to a state-of-the-art high performance-computing cluster maintained by the Biochemistry HPC core.
The Facility is managed by Maureen Donlin, Ph.D. (314-977-8858; firstname.lastname@example.org).
Recent publications from data analyzed by the core: