A shared-use facility that supports expression, purification, and analysis of research proteins by providing an array of instrumentation consultation on strategies for protein production from small to large scale. Available equipment includes shaking incubators, a New Brunswick Celligen fermentor, Avestin Emulsiflex cell disruptor, AKTA Purifier chromatograph, FlexStand hollow fiber concentrator, analytical ultracentrifuge, Beckman-Coulter analytical HPLC, and a Maxwell16 DNA/RNA/protein extraction system. Contact David Wood (email@example.com) for more information.
A recent addition to the Protein Core lab is the Shimadzu AXIMA Resonance™ MALDI-QIT-TOF mass spectrometer. The AXIMA Resonance™ is intended to be a workhorse instrument for protein and peptide characterization through its unique combination of MALDI sample introduction with a Quadrupole Ion Trap and high performance reflectron Time of Flight mass analyzer. In addition to peptide and protein analyses and identification, the instrument permits MS/MS spectra of a wide range of analytes, including pharmaceutical compounds, glycans, lipids, and polymers. MS/MS can be performed on singly charged precursor ions as high as 6-7 kDa.
The Resonance™ has been installed and is in testing mode. Training sessions will be scheduled in the near future on sample preparation, data collection, and interpretation. Instrument use charges for SLU researchers are in the process of cost-analysis.
A state-of-the-art X-ray facility is available to researchers in the department for the collection of high-resolution data on macromolecular samples. The facility has a 0.8 kW MM007 Rigaku generator with VHF optics interfaced to an R-Axis IV++ imaging plate system and a 1.2 kW MM007HF Rigaku generator with VHF optics double ported with two R-Axis IV++ imaging plate systems. A Phoenix robotic instrumentation from Art Robbins Instruments is available for the preparation, screening and imaging of crystals.
State-of-the-art differential scanning and isothermal titration calorimeters are available to researchers in the department to study protein stability and the thermodynamic components of protein-protein, protein-ligand and protein-nucleic acids interactions. These instruments include a MicroCal iTC200 microcalorimeter for isothermal titration studies and a MicroCal VP-DSC for differential scanning calorimetry.